abstract：:The survival of and interactions between a Pseudomonas putida strain labelled with a red fluorescent protein gene (WT-rfp) and its green fluorescent protein gene-labelled rpoS(-) mutant (KO-gfp) were examined. The generation times of the planktonic WT-rfp and KO-gfp in trypticase soy broth were not significantly diffe...

journal_title：Canadian journal of microbiology

authors： Maki ML,Lawrence JR,Swerhone GD,Leung KT

更新日期：2009-10-01 00:00:00

abstract：:Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, ho...

journal_title：Canadian journal of microbiology

authors： Pileggi SA,Vieira de Oliveira SF,Andrade CW,Vicente VA,Dalzoto Pdo R,Kniphoff da Cruz G,Gabardo J,Massola NS,Tozze HJ,Pileggi M,Kava-Cordeiro V,Galli-Terasawa LV,Pimentel IC,Stringari D,Glienke C

更新日期：2009-09-01 00:00:00

abstract：:Bacillus subtilis fmbR-1 was obtained from wild-type B. subtilis fmbR by replacement of the native promoter of the surfactin operon with the inducible promoter Pspac. The recombinant B. subtilis fmbR-1 produced more antibacterial substances than the wild-type strain. The overproducing phenotype was related to the enha...

journal_title：Canadian journal of microbiology

authors： Sun H,Bie X,Lu F,Lu Y,Wu Y,Lu Z

更新日期：2009-08-01 00:00:00

abstract：:The altitudinal distribution of microfungi associated with Betula ermanii Cham. leaf litter was investigated on Mt. Rishiri (1719 m), northern Japan. Fallen leaves were collected at five sites of different altitudes (approximately 300, 600, 900, 1200, and 1500 m above sea level) along a transect on the northern slope ...

journal_title：Canadian journal of microbiology

authors： Osono T,Hirose D

更新日期：2009-07-01 00:00:00

abstract：:Staphylococcus aureus isolated from the cervix of an infertile woman was found to cause complete immobilization of human spermatozoa in vitro. Only the cell culture and cell-free supernatant showed immobilization activity, indicating that the sperm immobilization factor might be released extracellularly by the organis...

journal_title：Canadian journal of microbiology

authors： Prabha V,Gupta T,Kaur S,Kaur N,Kala S,Singh A

更新日期：2009-07-01 00:00:00

abstract：:The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...

journal_title：Canadian journal of microbiology

authors： Pereira EM,Silva-Queiroz SR,Cabrera Gomez JG,Silva LF

更新日期：2009-06-01 00:00:00

abstract：:The search for new antimicrobial compounds involves finding novel sources of chemotherapeutic compounds or manipulating and combining structures from existing molecules. Small antimicrobial peptides (AMPs) are components of innate immune defenses characterized in greatest detail in insect-derived AMPs. We have generat...

journal_title：Canadian journal of microbiology

authors： Plunkett RM,Murray SI,Lowenberger CA

更新日期：2009-05-01 00:00:00

abstract：:In this study, 16S rRNA gene primers were designed to complement the suite of already available PCR primers for the detection of different methanogens involved in biogas production through anaerobic digestion by SYBR Green real-time PCR. Primers designed for use in TaqMan real-time PCR for the organisms Methanosaeta, ...

journal_title：Canadian journal of microbiology

authors： Franke-Whittle IH,Goberna M,Insam H

更新日期：2009-05-01 00:00:00

abstract：:beta-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using...

journal_title：Canadian journal of microbiology

authors： Venkateshwar M,Chaitanya K,Altaf MD,Hameeda B,Ghopal Reddy M

更新日期：2009-04-01 00:00:00

abstract：:Repetitive element-polymerase chain reaction (REP-PCR) DNA fingerprinting and library-based microbial source tracking (MST) methods were utilized to investigate the potential sources of Escherichia coli pollution in recreational waters of southeastern Lake Huron. In addition to traditional sources such as humans, agri...

journal_title：Canadian journal of microbiology

authors： Kon T,Weir SC,Howell ET,Lee H,Trevors JT

更新日期：2009-03-01 00:00:00

abstract：:The catalytic subunit of many Escherichia coli redox enzymes bares a twin-arginine translocation (Tat)-dependent signal peptide in its precursor, which directs the redox enzyme complex to this Sec-independent pathway. NarG of the E. coli nitrate reductase NarGHI complex possesses a vestige twin-arginine motif at its N...

journal_title：Canadian journal of microbiology

authors： Li H,Turner RJ

更新日期：2009-02-01 00:00:00

abstract：:Investigations of microbial diversity in Antarctic are important to begin to understand ecosystem functioning and decomposition processes. This study documents fungi at 9 historic sites on the Antarctic Peninsula collected from wooden structures, other organic materials, and soils during a joint National Science Found...

journal_title：Canadian journal of microbiology

authors： Arenz BE,Blanchette RA

更新日期：2009-01-01 00:00:00

abstract：:Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted ...

journal_title：Canadian journal of microbiology

authors： Zeng J,Yang LY,Liang Y,Li JY,Xiao L,Jiang LJ,Zhao DY

更新日期：2008-12-01 00:00:00

abstract：:HPr is a protein of the phosphoenolpyruvate:sugar phosphotransferase transport system (PTS). In gram-positive bacteria, HPr can be phosphorylated on Ser-46 by the kinase/phosphorylase HprK/P and on His-15 by phospho-enzyme I (EI~P) of the PTS. In vitro studies with purified HPrs from Bacillus subtilis, Enterococcus fa...

journal_title：Canadian journal of microbiology

authors： Roy DJ,Casabon I,Vaillancourt K,Huot JL,Vadeboncoeur C

更新日期：2008-11-01 00:00:00

abstract：:Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 degrees C and at a pH range of 6-11. The enzyme could be inhibited by azide, hydr...

journal_title：Canadian journal of microbiology

authors： Wang W,Sun M,Liu W,Zhang B

更新日期：2008-10-01 00:00:00

abstract：:Pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including Terminalia arjuna, Terminalia chebula, Azadirachta indica, and Holarrhena antidysenterica. Thirty Pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. The anti...

journal_title：Canadian journal of microbiology

authors： Tejesvi MV,Kini KR,Prakash HS,Subbiah V,Shetty HS

更新日期：2008-09-01 00:00:00

abstract：:The B subunit of Escherichia coli heat-labile enterotoxin (LTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral adjuvant that induces a strong immune response against co-administered or coupled antigens. In this paper, the effect of LTB on the humoral immune response to rec...

journal_title：Canadian journal of microbiology

authors： da Silva Ramos Rocha A,Conceição FR,Grassmann AA,Lagranha VL,Dellagostin OA

更新日期：2008-08-01 00:00:00

abstract：:Helicobacter pylori exists in 2 distinct morphological states, helicoid and coccoid. Both have been observed in in vitro culture and in gastric biopsies. We visualized H. pylori during AGS cell infections using immunofluorescence microscopy. Anti-H. pylori mouse serum as well as human serum from H. pylori-positive pat...

journal_title：Canadian journal of microbiology

authors： Couturier MR,Stein M

更新日期：2008-07-01 00:00:00

abstract：:When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests incre...

journal_title：Canadian journal of microbiology

authors： Ncango DM,Swart CW,Goldblatt ME,Pohl CH,Van Wyk PW,Botes PJ,Kock JL

更新日期：2008-06-01 00:00:00

abstract：:The diversity and variation of total and active ammonia-oxidizing bacteria in a full-scale aerated submerged biofilm reactor for drinking water pretreatment were characterized by clone libraries and denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA and its gene during a whole year. Sequences obtained...

journal_title：Canadian journal of microbiology

authors： Zhang XW,Qin YY,Ren HQ,Li DT,Yang H

更新日期：2008-05-01 00:00:00

abstract：:The structures of lipopolysaccharides (LPSs) of lic1 and lic1/lic2 mutants from Haemophilus influenzae type b strain Eagan (RM153) were investigated using methylation analysis, electrospray ionization - mass spectrometry, and nuclear magnetic resonance spectroscopy on O-deacylated, O- and N-deacylated core oligosaccha...

journal_title：Canadian journal of microbiology

authors： Masoud H,Moxon ER,Richards JC

更新日期：2008-04-01 00:00:00

abstract：:Streptococcus pneumoniae infection starts from the respiratory tract where interaction with host epithelial cells occurs. To gain more insights on pneumococcal pathogenesis, an oligonucleotide (oligo)-based microarray was used to investigate gene expression changes of one serotype 3 encapsulated pathogenic S. pneumoni...

journal_title：Canadian journal of microbiology

authors： Song XM,Connor W,Jalal S,Hokamp K,Potter AA

更新日期：2008-03-01 00:00:00

abstract：:Using a well tested antibody specific for 3-hydroxy oxylipins, we mapped the presence of these oxylipins in selected Cryptococcus (Filobasidiella) species. Immunofluorescence microscopy studies revealed that these compounds are deposited on cell wall surfaces, appendages, and collarettes. In vitro studies revealed tha...

journal_title：Canadian journal of microbiology

authors： Sebolai OM,Pohl CH,Botes PJ,van Wyk PW,Mzizi R,Swart CW,Kock JL

更新日期：2008-02-01 00:00:00

abstract：:Campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. No adhesins specific for C. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. Scanning electron microscopy revealed C. fetus attachment to the serosal surface...

journal_title：Canadian journal of microbiology

authors： Graham LL,Friel T,Woodman RL

更新日期：2008-01-01 00:00:00

abstract：:We examined isolates from 4 commercial bioinsecticides based on different strains of Bacillus thuringiensis subspecies (kurstaki, israelensis, aizawai, and tenebrionis) for the presence of genes encoding proteins with known enterotoxigenicity (nhe, hbl, cytk, ces) and various other putative virulence genes (piplc, sph...

journal_title：Canadian journal of microbiology

authors： Kyei-Poku G,Gauthier D,Pang A,van Frankenhuyzen K

更新日期：2007-12-01 00:00:00

abstract：:The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...

journal_title：Canadian journal of microbiology

authors： Lencáková D,Stefancíková A,Ivanová R,Petko B

更新日期：2007-12-01 00:00:00

abstract：:Culture-independent, biomarker-targeted bacterial enumeration and identification strategies were employed to estimate total bacterial burden and diversity within the cabin air of commercial airliners. Samples from each of 4 flights on 2 commercial carriers were collected via air-impingement. The total viable microbial...

journal_title：Canadian journal of microbiology

authors： La Duc MT,Stuecker T,Venkateswaran K

更新日期：2007-11-01 00:00:00

abstract：:This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...

journal_title：Canadian journal of microbiology

authors： D'Elia TV,Cooper CR,Johnston CG

更新日期：2007-10-01 00:00:00

abstract：:The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined. The enzyme was detected mainly in the membrane fraction. It catalyzed the sulfide-dependent menaquinone (MK) reduction showing special enz...

journal_title：Canadian journal of microbiology

authors： Shibata H,Suzuki K,Kobayashi S

更新日期：2007-09-01 00:00:00

abstract：:The goal of this study was to assess the presence and surfactant potential of naturally occurring microbes from a tropical soil with petrochemical contamination. Microorganisms in a soil sample from a Brazilian landfarm were isolated and grown on petroleum as the sole carbon source. Of 60 isolates screened for petrole...

journal_title：Canadian journal of microbiology

authors： Maciel BM,Dias JC,Dos Santos AC,Filho RC,Fontana R,Loguercio LL,Rezende RP

更新日期：2007-08-01 00:00:00

abstract：:Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography ...

journal_title：Canadian journal of microbiology

authors： Buzzini P,Innocenti M,Turchetti B,Libkind D,van Broock M,Mulinacci N

更新日期：2007-08-01 00:00:00

abstract：:In the present study, the effect of temperature and water activity on fungal growth and ochratoxin production on coffee-based medium was assessed. Optimal growth of three Aspergillus strains was observed in the same ecological conditions, namely 30 degrees C and 0.99 water activity. Maximal daily growth is 11.2, 6.92,...

journal_title：Canadian journal of microbiology

authors： Kouadio AI,Lebrihi A,Agbo GN,Mathieu F,Pfohl-Leszkowiz A,Dosso MB

更新日期：2007-07-01 00:00:00

abstract：:No clear data are available on how flavonoids from different chemical groups affect root colonization by arbuscular mycorrhizal fungi (AMF) and whether flavonoids affecting the presymbiotic growth of AMF also affect root colonization by AMF. In the present work, we compared the effect of flavones (chrysin and luteolin...

journal_title：Canadian journal of microbiology

authors： Scervino JM,Ponce MA,Erra-Bassells R,Bompadre J,Vierheilig H,Ocampo JA,Godeas A

更新日期：2007-06-01 00:00:00

abstract：:Fecal coliforms (FC) counts were compared with Escherichia coli counts in differently contaminated freshwater samples (n = 166). FC were enumerated by plate count on triphenyl 2,3,5-tetrazolium chloride Tergitol medium. Escherichia coli were enumerated by the most probable number microplate method based on the detecti...

journal_title：Canadian journal of microbiology

authors： Garcia-Armisen T,Prats J,Servais P

更新日期：2007-06-01 00:00:00

abstract：:The purpose of this study was to determine the prevalence of antibiotic-resistant bacteria and endotoxin in soil after land application of biosolids. Soil was collected over a 15 month period following land application of biosolids, and antibiotic resistance was ascertained using clinically relevant antibiotic concent...

journal_title：Canadian journal of microbiology

authors： Brooks JP,Maxwell SL,Rensing C,Gerba CP,Pepper IL

更新日期：2007-05-01 00:00:00

abstract：:The cellular functions are strongly influenced by the composition of the environment. In particular, phenotypes of microbial strains are modulated by concentrations of ions in the culture medium, and differences in element levels may be responsible for a phenotypic variability observed when microbial strains are grown...

journal_title：Canadian journal of microbiology

authors： Abelovska L,Bujdos M,Kubova J,Petrezselyova S,Nosek J,Tomaska L

更新日期：2007-04-01 00:00:00

abstract：:Dark spots disease (DSD) is a relatively new coral disease that has become one of the most prevalent afflictions in the Caribbean Sea. To partially characterize bacterial communities associated with DSD, carbon utilization patterns of bacterial strains isolated from the surface mucopolysaccharide layers of healthy and...

journal_title：Canadian journal of microbiology

authors： Gil-Agudelo DL,Fonseca DP,Weil E,Garzón-Ferreira J,Smith GW

更新日期：2007-04-01 00:00:00

abstract：:The possibility of using the volatile-producing fungus Muscodor albus for biofumigation against building molds was investigated. Several species of Aspergillus and Penicillium as well as fungi belonging to nine other genera were inhibited or killed in vitro by volatiles produced by potato dextrose agar or rye grain cu...

journal_title：Canadian journal of microbiology

authors： Mercier J,Jiménez JI

更新日期：2007-03-01 00:00:00

abstract：:Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two ...

journal_title：Canadian journal of microbiology

authors： Barberio C,Bianchi L,Pinzauti F,Lodi T,Ferrero I,Polsinelli M,Casalone E

更新日期：2007-02-01 00:00:00

abstract：:Uroporphyrinogen decarboxylase (UroD) (EC 4.1.1.37) is an enzyme from the tetrapyrrole biosynthetic pathway, in which chlorophyll is the main final product in algae. This is the first time that a study on UroD activity has been performed in a green alga (Chlorella). We isolated and partially purified the enzyme from a...

journal_title：Canadian journal of microbiology

authors： Juárez AB,Aldonatti C,Vigna MS,Ríos de Molina Mdel C

更新日期：2007-02-01 00:00:00